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KMID : 0364820220580010012
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Korean Journal of Microbiology
2022 Volume.58 No. 1 p.12 ~ p.22
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Characterization of fermentation bacteria isolated from horse and wild roe deer feces in Jeju Island using the culture-dependent method§
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Lee Myung-Lip
Jung Man-Young
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Abstract
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Jeju Island, created by volcanic activity, has a specific natural ecosystem and various creatures, different from the mainland in Korea. In this study, we collected feces samples of horses (hindgut fermentor) and wild roe deer (foregut fermentor) living in Jeju Island to identify the diversity of fermentation bacteria by the culture defendant method. The total 17 bacterial strains (genus Acinetobacter, Arthrobacter, Bacillus, Brachybacterium, Calidifontibacter, Enterococcus, Escherichia, Kocuria, Micrococcus, Oerskovia, Rothia, Staphylococcus, Streptococcus) were identified and isolated in the growth of lactic acid bacteria-specific media. The similarity of the 16S rRNA gene sequence of the isolated bacterial and yeast strains excluded a bacterial strain to those of reported strains was > 99%. A bacterial strain, Bracybacterium sp. isolated from horse feces, was a 97.85% similarity with Brachybacterium nesterenkovii strain, and it is a candidate of novel species in the same genus. In the acid production test with the addition of four different saccharides, it has been identified that the strains isolated from feces of wild roe deer have higher fermentation activity than strains from the horse at anaerobic conditions. Our results indicate that various microorganisms with fermentation activity were collected from Jeju horses and wild roe deer feces by the culture-dependent method. Therefore, it is worth checking the production yield in various growth conditions and identifying the fermentation product in future studies to apply the isolated microbial strains.
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KEYWORD
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anaerobic, culture-dependent method, fermentation, horse, Jeju island, Roe deer
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